abstract：:The structural organization of cellular membranes has an essential influence on their functionality. The membrane surfaces currently are considered to consist of various distinct patches, which play an important role in many processes, however, not all parameters such as size and distribution are fully determined. In ...

journal_title：Journal of biophotonics

authors： Deckert-Gaudig T,Böhme R,Freier E,Sebesta A,Merkendorf T,Popp J,Gerwert K,Deckert V

更新日期：2012-07-01 00:00:00

abstract：:An integrated multimodal optical microscope is demonstrated for high-resolution, structural and functional imaging of engineered and natural skin. This microscope incorporates multiple imaging modalities including optical coherence (OCM), multi-photon (MPM), and fluorescence lifetime imaging microscopy (FLIM), enablin...

journal_title：Journal of biophotonics

authors： Zhao Y,Graf BW,Chaney EJ,Mahmassani Z,Antoniadou E,Devolder R,Kong H,Boppart MD,Boppart SA

更新日期：2012-05-01 00:00:00

abstract：:In this work we present how to entirely remove the scattering ambiguity present in existing multiphoton multifocal systems. This is achieved through the development and implementation of single-element detection systems that incorporate high-speed photon-counting electronics. These systems can be used to image entire ...

journal_title：Journal of biophotonics

authors： Hoover EE,Field JJ,Winters DG,Young MD,Chandler EV,Speirs JC,Lapenna JT,Kim SM,Ding SY,Bartels RA,Wang JW,Squier JA

更新日期：2012-05-01 00:00:00

abstract：:Conventional histopathology is rapidly shifting towards digital integration. Will microscopes (and pathologists) soon be obsolete? Or are we dealing with just another image modality that leaves the core of tissue diagnosis intact? This article provides an overview of current digital pathology applications and research...

journal_title：Journal of biophotonics

authors： Brachtel E,Yagi Y

更新日期：2012-04-01 00:00:00

abstract：:Mucosal lesions of the upper aerodigestive tract usually concern the squamous epithelium of the surface. Many precancerous alterations now designated as potentially malignant are caused by DNA damage resulting from long term exposure to tobacco and alcohol consumption. The distinction from conditions with a similar ph...

journal_title：Journal of biophotonics

authors： Dreyer T,Kreisel M

更新日期：2012-04-01 00:00:00

abstract：:We describe a novel, minimally invasive laser technology for skin rejuvenation by creating isolated microscopic lesions within tissue below the epidermis using laser induced optical breakdown. Using an in-house built prototype device, tightly focused near-infrared laser pulses are used to create optical breakdown in t...

journal_title：Journal of biophotonics

authors： Habbema L,Verhagen R,Van Hal R,Liu Y,Varghese B

更新日期：2012-02-01 00:00:00

abstract：:We suggest to use a combination of optical tweezers and single-image quantitative differential interference contrast (DIC) emulated by a spatial light modulator (SLM) to study physiological shape changes in thrombocytes after activation and demonstrate the effectiveness of this system for the given task. A specially d...

journal_title：Journal of biophotonics

authors： Khan S,Jesacher A,Nussbaumer W,Bernet S,Ritsch-Marte M

更新日期：2011-09-01 00:00:00

abstract：:The capabilities of cross-polarization optical coherence tomography (CP OCT) for early bladder-cancer detection are assessed in statistical study and compared with the traditional OCT. Unlike the traditional OCT that demonstrates images only in copolarization, CP OCT acquires images in cross-polarization and copolariz...

journal_title：Journal of biophotonics

authors： Gladkova N,Streltsova O,Zagaynova E,Kiseleva E,Gelikonov V,Gelikonov G,Karabut M,Yunusova K,Evdokimova O

更新日期：2011-08-01 00:00:00

abstract：:We developed an optical imaging method based on a feedback principle in which the specific scan pattern is adapted according to the shape of the sample. The feedback approach produces nanometer-resolved 3D images of very small and moving features in live cells in seconds. We show images of microvilli in live cultured ...

journal_title：Journal of biophotonics

authors： Lanzano L,Digman MA,Fwu P,Giral H,Levi M,Gratton E

更新日期：2011-06-01 00:00:00

abstract：:The photodetachment of Br(-), I(-) and OH(-) in aqueous solution is studied by 2- and 3-pulse femtosecond spectroscopy. The UV excitation leads to fast electron separation followed by formation of a donor-electron pairs. An additional repump pulse is used for secondary excitation of the intermediates. The 3-pulse tech...

journal_title：Journal of biophotonics

authors： Fischer MK,Gliserin A,Laubereau A,Iglev H

更新日期：2011-03-01 00:00:00

abstract：:In this paper we introduce a custom scanning near-field optical microscope (SNOM) that simultaneously collects reflection and transmission near-field images along with topography. This dual-optical SNOM uses a bent probe, which allows for axial reflection imaging, accurate surface scanning, and easy identification of ...

journal_title：Journal of biophotonics

authors： Kyle JR,Kyle MD,Raghavan R,Budak G,Ozkan CS,Ozkan M

更新日期：2011-03-01 00:00:00

abstract：:DsRed is representative of the tetrameric reef coral fluorescent proteins that constitute particularly interesting coupled multichromophoric systems. Either a green emitting or a red emitting chromophore can form within each of the monomers of the protein tetramer. Within the tetramers the chromophores are thought to ...

journal_title：Journal of biophotonics

authors： Blum C,Meixner AJ,Subramaniam V

更新日期：2011-01-01 00:00:00

abstract：:The processes associated with pollen germination were studied in vitro for two tree species, Salix caprea and Fraxinus excelsior under different nutrient conditions. The results provide evidence of changes in chemical composition of the pollen grains during germination. From the comparison of spectra of the pollen gra...

journal_title：Journal of biophotonics

authors： Schulte F,Panne U,Kneipp J

更新日期：2010-08-01 00:00:00

abstract：:For a precise manipulation of particles and cells with laser light as well as for the understanding and the control of the underlying processes it is important to visualize and quantify the response of the specimens. Thus, we investigated if digital holographic microscopy (DHM) can be used in combination with microflu...

journal_title：Journal of biophotonics

authors： Kemper B,Langehanenberg P,Höink A,von Bally G,Wottowah F,Schinkinger S,Guck J,Käs J,Bredebusch I,Schnekenburger J,Schütze K

更新日期：2010-07-01 00:00:00

abstract：:The authors have developed a tomographic diffractive microscope that combines microholography with illumination from an angular synthetic aperture. It images specimens relative to their complex index of refraction distribution (index and absorption) and permits imaging of unlabelled specimens, with high lateral resolu...

journal_title：Journal of biophotonics

authors： Simon B,Debailleul M,Beghin A,Tourneur Y,Haeberlé O

更新日期：2010-07-01 00:00:00

abstract：:Photodynamic inactivation of bacteria (PDIB) is considered a new approach for the struggle against multiresistant bacteria. To achieve a sufficient level of bacteria killing, the photosensitizer must attach to and/or penetrate the bacteria and generate a sufficiently high amount of singlet oxygen. To optimize PDIB, th...

journal_title：Journal of biophotonics

authors： Regensburger J,Maisch T,Felgenträger A,Santarelli F,Bäumler W

更新日期：2010-06-01 00:00:00

abstract：:Lasers are used in dentistry for a variety of indications. One of these is the disinfection of root canals or the sterilization of residual caries. Many studies have demonstrated the capacity to kill bacteria for lasers but the fundamental mechanism of the laser effect remains quite unclear. With our experiments we wa...

journal_title：Journal of biophotonics

authors： Hibst R,Graser R,Udart M,Stock K

更新日期：2010-06-01 00:00:00

abstract：:Light delivery and monitoring during photodynamic therapy (PDT) is often limited by the need for a physical link between the light source, detectors and the treatment volume. This paper reports on the first in vivo experiments performed with a fully implantable telemetric system, designed for a rat glioblastoma model....

journal_title：Journal of biophotonics

authors： van Zaane F,Subbaiyan D,van der Ploeg-van den Heuvel A,de Bruijn HS,Balbas EM,Pandraud G,Sterenborg HJ,French PJ,Robinson DJ

更新日期：2010-06-01 00:00:00

abstract：:A murine model of cutaneous leishmaniasis with green fluorescent protein positive (GFP+) L. major enables the monitoring of parasitic load via measurements of GFP fluorescence intensity, allowing for a faster and more efficient way of monitoring the clinical outcome of photodynamic therapy (PDT). This model may provid...

journal_title：Journal of biophotonics

authors： Latorre-Esteves E,Akilov OE,Rai P,Beverley SM,Hasan T

更新日期：2010-06-01 00:00:00

abstract：:A Monte Carlo simulation was adapted to specify a skin model with pigmented epidermis, dermis with low baseline blood content, and vessels of a vascular lesion with an average added blood volume fraction of 5%. In the study, the lesion vessel diameters were increased and the number of lesion vessels decreased, such th...

journal_title：Journal of biophotonics

authors： Jacques SL

更新日期：2010-01-01 00:00:00

abstract：:Quantification of the mucosa oxygenation levels during Endoscopic imaging provides useful physiological/diagnostic information. In this work a method for non-contact quantification of the oxygen saturation index during Endoscopic imaging using three discrete spectral-band in the blue, the green, and the red parts of t...

journal_title：Journal of biophotonics

authors： Fawzy Y

更新日期：2009-12-01 00:00:00

abstract：:Highly emissive europium complexes with specific endoplasmic reticulum localization potential includes several advantages such as fast uptake, long resident lifetime, low dosage requirement, low cytotoxicity and highly emissive two-photon induced f-f emission imaging. ...

journal_title：Journal of biophotonics

authors： Law GL,Wong KL,Man CW,Tsao SW,Wong WT

更新日期：2009-12-01 00:00:00

abstract：:In this review we provide a description of the basic concepts and paradigms currently constituting the foundations of adult stem cell biology, and discuss the role that live imaging techniques have in the development of the field. We focus on live imaging of hematopoietic stem cells (HSCs) as the basic biology and cli...

journal_title：Journal of biophotonics

authors： Lo Celso C,Wu JW,Lin CP

更新日期：2009-11-01 00:00:00

abstract：:As the field of tissue engineering evolves, there will be an increasingly important need to visualize and track the complex dynamic changes that occur within three-dimensional constructs. Optical coherence tomography (OCT), as an emerging imaging technology applied to biological materials, offers a number of significa...

journal_title：Journal of biophotonics

authors： Liang X,Graf BW,Boppart SA

更新日期：2009-11-01 00:00:00

abstract：:Initially, it has been shown that the phase thickness and refractivity (the latter interpreted as the difference of the refractivity indices of an object and surrounding medium) depend on the functional state of mitochondria. The refractivity of various objects decreased in response to energy depletion. This dependenc...

journal_title：Journal of biophotonics

authors： Tychinsky V

更新日期：2009-09-01 00:00:00

abstract：:Optical spectroscopy of a single gold nanoparticle, functionalized with an aptamer, is used to sense the specific binding of avidin. Herewith, the field of single noble metal nanoparticle biosensors is extended to the important field of aptamer based assays. The sensitivity of this initial, but not yet optimized apta-...

journal_title：Journal of biophotonics

authors： Hernandez FJ,Dondapati SK,Ozalp VC,Pinto A,O'Sullivan CK,Klar TA,Katakis I

更新日期：2009-04-01 00:00:00

abstract：:In this work we aim to show how it is possible to exploit the second harmonic generation (SHG) signal for producing multimodal microscopic images of biological tissues. SHG microscopy constitutes an important tool for high-resolution, high-contrast, three-dimensional studies of live cell and tissue architectures. The ...

journal_title：Journal of biophotonics

authors： Bianchini P,Diaspro A

更新日期：2008-12-01 00:00:00

abstract：:Microfluidics and photonics come together to form a field commonly referred to as 'optofluidics'. Flow cytometry provides the field with a technology base from which both microfluidic and photonic components be developed and integrated into a useful device. This article reviews some of the more recent developments to ...

journal_title：Journal of biophotonics

authors： Godin J,Chen CH,Cho SH,Qiao W,Tsai F,Lo YH

更新日期：2008-10-01 00:00:00

abstract：:Molecular motors have been extensively studied over the past 25 years using a range of optical microscopy methods. The specific example of RNA polymerase, responsible for transcribing the information encoded in DNA into RNA, illustrates the strength of the convergence between these recent approaches and the vast store...

journal_title：Journal of biophotonics

authors： Strick TR

更新日期：2008-09-01 00:00:00

abstract：:The advent of moderate dilatations in ascending aortas is often accompanied by structural modifications of the main components of the aortic tissue, elastin and collagen. In this study, we have undertaken an approach based on FTIR microscopy coupled to a curve-fitting procedure to analyze secondary structure modificat...

journal_title：Journal of biophotonics

authors： Bonnier F,Rubin S,Debelle L,Ventéo L,Pluot M,Baehrel B,Manfait M,Sockalingum GD

更新日期：2008-08-01 00:00:00